Cells, Free Full-Text
Por um escritor misterioso
Descrição
Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimal OFMC growth was observed in 20% fetal bovine serum at 28 °C with 0.8 mM CaCl2 and the basic fibroblast growth factor (BFGF) to enhance cell growth. OFMCs have become permanent cell lines through the spontaneous immortalization crisis at the 20th passage. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMCs.
Towards reproducible cell-free systems
Frontiers Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
Antibodies, Free Full-Text
Cancer type classification using plasma cell-free RNAs derived from human and microbes
Remote immune processes revealed by immune-derived circulating cell-free DNA
Cells, Free Full-Text
Nt Novo Cella Get File - Colaboratory
Cell-free Macromolecular Synthesis
Cell Circuits and Complex Tissues
Advances and applications of cell-free systems for metabolic production - ScienceDirect
Cells, Free Full-Text
de
por adulto (o preço varia de acordo com o tamanho do grupo)
/i.s3.glbimg.com/v1/AUTH_59edd422c0c84a879bd37670ae4f538a/internal_photos/bs/2019/G/r/qiIpNeSZCcahR0M0W1eQ/ap19198145465190.jpg)
