Metabolic engineering of Deinococcus radiodurans for pinene
Por um escritor misterioso
Descrição
Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.
Loop Carsten Jers
Research Progress of Plant Prenyltransferases
Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance
A Novel Small RNA, DsrO, in Deinococcus radiodurans Promotes Methionine Sulfoxide Reductase (msrA) Expression for Oxidative Stress Adaptation
Biosynthesis, evolution and ecology of microbial terpenoids - Natural Product Reports (RSC Publishing) DOI:10.1039/D1NP00047K
Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories, Bioresources and Bioprocessing
PDF) G19. Metabolic Engineering of E. col i for Regiospecific Modifications of Naringenin to Produce Astragalin
PDF] Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene.
Bacterial Production of Pinene by a Laboratory-Evolved Pinene-Synthase
Microbial Synthesis of Pinene
Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance
Microbial Synthesis of Pinene
Listening to enteric bacteria from the perspective of antibiotic alternatives in animal husbandry
PDF) (Volume 2) Plant Secondary Metabolites, Volume Two Stimulation, Extraction, and Utilization
Engineering Robustness of Microbial Cell Factories - Gong - 2017 - Biotechnology Journal - Wiley Online Library
de
por adulto (o preço varia de acordo com o tamanho do grupo)
